Loma Linda University

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Jun Wang, MD
Professor, Pathology and Human Anatomy
School of Medicine
Publications    Scholarly Journals--Published
  • X.H. Zhang, S. Kelaria, J. Kerstetter, J. Wang. The functional and prognostic implications of regulatory T cells in colorectal cancer. J Gastrointestinal Oncol. 2015;6:307-313. doi: 10.3978/j.issn.2078-6891.2015.017 ( 6/2015 )
  • B. Wang, B.B. Song, O. Cyrus, J.D., Cao, A. Raza, J. Wang. Coexistence of intestinal Kaposi sarcoma and plasmablastic lymphoma in an HIV/AIDS patient: case report and review of the literature. J Gastrointestinal Oncol. 2015  doi: 10.3978/j.issn.2078-6891.2015.021 ( 6/2015 )
  • B. Li, D.J. Baylink, M.H. Walter, K.H. Lau, C. Deb, X. Meng, J. Wang, X. Qin. Targeted 25-Hydroxyvitamin D3 1α hydroxylase adoptive gene therapy ameliorates DSS-induced colitis without causing hypercalcemia in mice. Mol. Ther. 2015;23:339-351. 2014 Oct 20. doi: 10.1038/mt.2014.201. [Epub ahead of print] ( 3/2015 )
  • Y. Wang, Q He, W. Shi, J. Wang, H.X. Ji. A mixture of carcinoid tumors, extensive neuroendocrine cell proliferation, and multiple pulmonary sclerosing hemangiomas. World J Surg Oncol.2014,12:209 http://www.wjso.com/content /12/1/209 ( 10/2014 )
  • J. Tellez, C. Jaing, J. Wang, R. Green, M.Y. Chen. Detection of Epstein-Barr virus (EBV) in human lymphoma tissue by a novel microbial detection array. Biomarker Research. 2014, 2:49/DOI: 10.1186/s40364-014-0024-x ( 9/2014 )
  • J.L. Smith, A. Kheradpour, C. Zuppan, J. Wang, R.P. Ketterling, E. Rowsell. Sequential development of precursor B-cell acute lymphoblastic leukemia and precursor T-cell lymphoblastic lymphoma. J Cancer Res Updates. 2014;3:117-122. DOI: http://dx.doi.org/10.6000/1929-2279.2014.03.02.6 ( 5/2014 )
  • E. Wang, P. Papavassiliou, A.P. Wang, A. Louissaint, Jr., J. Wang, C.B. Hutchinson, Q. Huang, D. Reddi, Q. Wei, S. Sebastian, C. Rehder, R. Brynes, Imran Siddiqi. Composite lymphoid neoplasm of B-cell and T-cell origins: a pathological study of 14 cases. Human Pathology. 2014;45:768-84. doi: 10.1016/j.humpath.2013.11.008. Epub 2013 Nov 26. ( 4/2014 )
  • C.L. Booth, J. Wang. Unexpected Malignancies In Routine Tonsillectomy Specimens: A Single Institutional Experience and Review of the Literature. Am J Clin Pathol. 2013;140:807-812. doi:10.1309/AJCPCM7LN4QEZPOQ. ( 12/2013 ) Link...
  • R.J. Su, D.J. Baylink, A. Neises, X. Meng, J.B. Kiroyan, K.J. Payne, Y. Duan, B. Tschudy-Seney, N. Appleby, M. Kearns-Jonker, D.S. Gridley, J. Wang, K.-H. W. Lau, X.B. Zhang. Efficient Generation of Integration-Free iPS Cells from Human Adult Peripheral Blood Using BCL-XL Together With Yamanaka Factors. PLoS ONE 8(5): e64496. doi:10.1371/journal.pone.0064496 (2013 Molecular Therapy.). ( 4/2013 ) Link...
  • J. Kerstetter, M. C. Perez, P. Herrmann, C. Zuppan, J. Wang. Epithelioid hemangioendothelioma with a prominent lymphoid infiltrate mimicking follicular dendritic cell tumor: report of a case. J Cancer Res Updates. 2013;2:135-139.  DOI: http://dx.doi.org/10.6000/1929-2279.2013.02.02.8 ( 4/2013 ) Link...
  • M.A. Bautista-Quach, C.L. Booth, A. Kheradpour, C.W. Zuppan, E.H. Rowsell, L.M. Weiss, J. Wang. Mast Cell Sarcoma in an Infant: A Case Report and Review of the Literature. J Ped Hematol Oncol. 2013;35:315-20. doi: 10.1097/MPH.0b013e318279e392. ( 3/2013 ) Link...
  • X. Meng, R.J. Su, D.J. Baylink, A. Neises, J.B. Kiroyan, W.Y.-W Lee, K.J. Payne, D. S. Gridley, J.  Wang, K.-H. W. Lau, G. Li and X.-B. Zhang. Rapid and Efficient Reprogramming of Human Fetal and Adult Blood CD34+ Cells into Mesenchymal Stem Cells with a Single Factor. Cell Research (advance online publication). March 12, 2013; doi:10.1038/cr.2013.40 ( 3/2013 ) Link...
  • X. Meng, A. Neises, R.J. Su, K.J. Payne, L. Ritter, D.S. Gridley, J. Wang, M. Sheng, K.-H. W. Lau, D.J. Baylink1, X.B. Zhang. Efficient reprogramming of human cord blood CD34+ cells into induced pluripotent stem cells with OCT4 and SOX2 alone. Molecular Therapy. 2012;20:408-416. doi: 10.1038/mt.2011.258. ( 2/2012 - 2/2013 ) Link...
  • M. Van Ness, J. Gregg, J. Wang, M. Chen. Genetics and Molecular Pathology of Gastric Malignancy: Development of Targeted Therapies in the Era of Personalized Medicine. J Gastrointestinal Oncol. 2012;3:182-188. DOI: 10.3978/j.issn.2078-6891.2012.017 ( 9/2012 ) Link...
  • M. Bautista-Quach, C. Ake, J. Wang. Gastrointestinal Lymphomas: Morphology, Immunophenotype, and Molecular Features. J Gastrointestinal Oncol. 2012;3;209-225. DOI: 10.3978/j.issn.2078-6891.2012.024. ( 9/2012 )
  • J. Wang. Pathologic aspects of tumors of gastrointestinal tract in the era of personalized medicine (Editorial). J. Gastrointestinal Oncol. 2012;3:151-152. ( 9/2012 )
  • M.A. Bautista-Quach, A. Bedros, J. Wang. Subsequent development of diffuse large B-cell lymphomas and Hodgkin lymphoma associated with primary immune disorder in a 6-year-old female: A case report and review of the literature. J Ped Hematol Oncol. 2011;33:e320-325. ( 5/2011 )
  • Y.S. Noronha, A.S. Raza, B. Hutchins, D.R. Chase, C.A. Garberoglio, P.G. Chu, L.M. Weiss, J. Wang. CD34, CD117 and Ki-67 expression in phyllodes tumor of the breast: an immunohistochemical study of 33 cases. Int J Surg Pathol. 2011;19:152-158. ( 4/2011 )
  • H. Wong, J. Wang. Merkel Cell Carcinoma. Arch Pathol Lab Med. 2010;134:1711-1716. ( 11/2010 )
  • S.H. Yi, G. An, J.Y. Qi, D.H.  Zou, Y.Z. Zhao, P.H. Zhang, H.S. Chen, J. Wang, L.Q. Qiu. The Significance of Marrow nvolvement In Aggressive Lymphomas: A Retrospective Comparison of Clinical Outcomes Between Peripheral T cell Lymphoma and Diffuse Large B cell Lymphoma. Acta Haematol. 2010;124:239-244. ( 11/2010 )
  • J. Wang, W.G. Saukel, C. Garberoglio, W. Srikureja, C-T. Hsueh. Pathologically complete response after neoadjuvant chemotherapy with trastuzumab-containing regimen in gastric cancer. J Hematol Oncol. 2010, 3:31  doi:10.1186/1756-8722-3-31. ( 9/2010 )
  • W. Chen, J. Wang, E. Wang , Y. Lu, S.K. Lau, L.M. Weiss,  Q. Huang. Detection of Clonal Lymphoid Receptor Gene Rearrangements in Langerhans Cell Histiocytosis. Am J Surg Pathol. 2010;34:1049-1057. ( 7/2010 )
  • J. Guo, J. Wang. Adult ocular xanthogranulomatous disease (AOXGD) (Review). Arch Pathol Lab Med. 2009;133:1994-1997. ( 12/2009 )
  • M. Bautista, W.D.Y. Quan, Jr., J. Wang. A case of chronic conjunctivitis following rituximab therapy. Advances in Hematology. Volume 2009, Article ID 272495, 4 pages, doi:10.1155/2009/272495. ( 7/2009 )
  • W. Chen, S.K. Lau, D. Fong, J. Wang, E. Wang, D.A. Arber, L.M. Weiss, Qin Huang. High Frequency of Clonal Immunoglobulin Receptor Gene Rearrangements in Sporadic Histiocytic/Dendritic Sarcomas. Am J Surg Pathol. 2009;33:863-873 ( 6/2009 )
  • H. Wong, J. Wang. EBV-positive diffuse large B-cell lymphoma of the elderly. Leuk. Lymphoma. 2009;50:335-340. ( 6/2009 )
  • W. Chen, Q. Huang, C. Zuppan, E.H. Rowsell, J.D. Cao, L.M. Weiss, J. Wang. Complete Absence of KSHV/HHV-8 in post-transplant lymphoproliferative disorders – an immunohistochemcial and molecular study of 52 cases. Am J Clin Pathol. 2009;131:632-639. ( 5/2009 )
  • D. Buxton, C.E. Bacchi, Lawrence M. Weiss, C. Zuppan, E.H. Rowsell, G. Gualco, Q. Huang, J. Wang. Frequent expression of CD99 in anaplastic large cell lymphoma – an immunohistochemical study of 160 cases. Am J Clin Pathol. 2009;131:574-579. ( 4/2009 )
  • J. Wang, C. Chen, S. Lau, R. Raghavan, E.H. Rowsell, J. Said, L.M. Weiss, Q. Huang. CD3-positive large B-cell lymphoma. Am J Surg Pathol. 2009;33:505-512. ( 3/2009 )
  • R. Agrawal, J. Wang. Pediatric Follicular Lymphoma. Arch Pathol Lab Med. 2009;133:142-146. ( 1/2009 )
  • M. Y. Chen, Jun Wang. Gaucher “Go-Shay” disease: review of the literatures.  Archives of Pathology & Laboratory Medicine 132. (2008): 851-853. ( 5/2008 )
  • A. Pham, J. Wang. "Bernard-Soulie Syndrome - An Inherited Bleeding Disorder (Review)." Archives of Pathology & Laboratory Medicine 131. (2007): 1834-1836. ( 12/2007 )
  • S. Pitman, J. Wang, C. Zuppan. "A 70-year-old woman with acute renal failure - crystal storing histiocytosis." Archives of Pathology & Laboratory Medicine 130.7 (2006): 1077-1078. ( 7/2006 )
  • B. Semakula, J. Rittenback, Jun Wang. "Hodgkin lymphoma-like posttransplantation lymphoproliferative disorder." Archives of Pathology and Laboratory Medicine 130.4 (2006): 558-560. ( 4/2006 ) Link...
    Posttransplantation lymphoproliferative disorders (PTLD) are a heterogeneous group of monoclonal or polyclonal lymphoproliferative lesions that occur in immunosuppressed recipients following solid organ or bone marrow transplantation, including 4 categories: (1) early lesions (reactive plasmacytic hyperplasia, and infectious-mononucleosis-like PTLD), (2) polymorphic PTLD, (3) monomorphic PTLD (including B-cell neoplasms and T-cell neoplasms), and (4) Hodgkin lymphoma (HL) and HL-like PTLD in the current World Health Organization classification. Although HL-like PTLD has been grouped with classic HL PTLD, controversy remains as to whether it is truly a form of HL or whether it should be more appropriately considered as a form of B-cell PTLD. The current available literature data indicate the presence of important immunophenotypic, molecular genetic, and clinical differences between HL PTLD and HL-like PTLD, suggesting that HL-like PTLD is in fact most often a form of B-cell PTLD. Distinction from true HL may be important for clinical management and prognosis.
  • S. Pitman, Q. Huang, C. Zuppan, E.H. Rowsell, J.D. Cao, J.G. Berdeja, L.M. Weiss, Jun Wang. "Hodgkin Lymphoma-like (HD-like) Post-Transplant Lymphoproliferative Disorder (PTLD) simulates monomorphic B-cell PTLD both Clinically and Pathologically." American Journal of Surgical Pathology 30.4 (2006): 470-470. ( 4/2006 ) Link...
    Although Hodgkin lymphoma-like posttransplantation lymphoproliferative disorder (HL-like PTLD) has been grouped with classic Hodgkin lymphoma type PTLD (HL-PTLD), controversy remains as to whether it is truly a form of HL or whether it should be more appropriately classified as a form of B-cell PTLD. Because only few cases of HL-like PTLD have been reported, their pathologic nature and clinical behavior have not been well defined. This report characterized 5 cases of HL-like PTLD with respect to their immunophenotype, EBV status, clonality, and clinical outcome. All of the patients were male, with ages ranging from 1.5 to 55 years at diagnosis. PTLD developed from 4 months to 6 years following solid organ transplantation (3 hearts, 1 kidney, 1 liver), and involved both nodal and extranodal sites. All were EBV-related (EBER+) with the large neoplastic cells CD20/CD79a positive but CD15 negative. Immunoglobulin gene rearrangements were detected in 3 of 5 tested. All patients were managed by initial reduction/withdrawal of immunosuppression, with 2 also receiving chemotherapy for non-HL. Three patients died of progressive disease within 2 to 3 months after diagnosis, 1 is alive and well 2 years later, and the fifth was disease free but died of unrelated causes (graft coronary disease) 2 years later. We conclude that, although HL-like PTLD morphologically simulates classic HL PTLD, there are important immunophenotypic, molecular genetic, and clinical differences, suggesting it is in fact most often a B-cell PTLD. Distinction between HL and HL-like PTLD may be important for clinical management and prognosis.
  • S. Pitman, A. Victorio, J.D. Morris, E.H. Rowsell, J.D. Cao, Jun Wang. "5q- syndrome in a child with slowly progressive pancytopenia. A case report and review of the literature." Journal of Pediatric Hematology and Oncology 28.3 (2006): 115-119. ( 3/2006 ) Link...
    5q- syndrome is a rare myelodysplastic process occurring predominately in middle aged to elderly women. In children, myelodysplasia of all types is rare and 5q- syndrome is exceptionally rare. Only 6 cases of 5q- associated myelodysplasia have been reported in children and all 6 cases had blast counts >5% and/or additional cytogenetic abnormalities. We report a case of 5q- syndrome in a girl who presented with macrocytosis and intermittent pancytopenia at age 5. Cytogenetic studies at age 8 revealed a large interstitial deletion of chromosome 5q without other cytogenetic abnormalities. The patient was clinically stable until age 11, when she became transfusion dependent and severely neutropenic. Subsequently, she underwent a successful unrelated cord blood transplant. To our knowledge, this is the first reported pediatric case meeting the strict criteria for 5q- syndrome.
  • N. Zekry, M.J. Klooster, R. Raghavan, Jun Wang. "A 7-year-old child with history of acute myeloid leukemia presenting with multiple gastrointestinal polyps." Archives of Pathology and Laboratory Medicine 130.1 (2006): e3-e4. ( 1/2006 ) Link...
  • J. Rittenbach, J.D. Cao, E.H. Rowsell, L.M. Weiss, W. Chick, Jun Wang. "Primary diffuse large B-cell lymphoma presented solely as an endometrial polyp." International Journal of Gynecological Pathology 24.4 (2005): 347-351. ( 10/2005 ) Link...
    We report a primary diffuse large B-cell lymphoma of endometrial polyp in a 44-year-old woman who presented with irregular vaginal spotting and was found to have a polyp protruding from the cervical os. Histology of the polyp showed an atypical diffuse infiltration by large, mononuclear cells within the stroma and between endometrial glands in one of the polypoid fragments. Immunohistochemistry and testing for immunoglobulin heavy chain gene rearrangement showed a B-cell lineage, consistent with diffuse large B-cell lymphoma. Staging procedures including detailed gynecology examination, body computed tomography scan, and bone marrow examination, as well as total hysterectomy, showed no evidence of lymphoma outside of the polyp. To our knowledge, this represents the first well-documented instance of primary lymphoma of the uterus presenting as an endometrial polyp. The differential diagnosis of endometrial biopsies containing an atypical lymphoid infiltrate should include the rather rare possibility of primary uterine lymphoma arising in an endometrial polyp. Immunohistochemistry and/or molecular analysis for antigen receptor gene rearrangements are critical in arriving at the correct diagnosis.
  • J. Wang, NCJ Sun, YY Chen, LM Weiss. "T cell/histiocyte-rich large B-cell Lymphoma Displays a Similar Heterogeneity as Regular Diffuse Large B Cell Lymphoma - A Clinicopathologic and Immunohistochemical Study of 30 Cases.." Applied Immunohistochemistry & Molecular Morphology 13.2 (2005): 109-115. ( 6/2005 ) Link...
    T-cell/histiocyte-rich large B-cell lymphoma (THRLBCL), a proliferating peripheral B-cell neoplasm, is a morphologic variant of diffuse large B-cell lymphoma (DLBCL), which may be confused with Hodgkin''s lymphoma, non-Hodgkin''s lymphoma, and reactive lymphadenopathies. Though more recent studies suggested that it might be a distinct clinicopathologic entity and/or a heterogeneous entity with derivation from germinal center B cells, its histogenetic derivation remains controversial. The authors analyzed 30 cases of THRLBCL to further characterize the origin of the neoplastic cells using immunohistochemical and molecular studies for expression of Bcl-6, CD10, and CD138, as well as rearrangements of IgH/bcl-2 genes on paraffin-embedded tissue. Half of the cases (15/30) showed Bcl-6 expression and five cases (19%) showed CD10 expression, but none had CD138 expression (0/20). Only three cases showed coexpression of both Bcl-6 and CD10. Molecular studies performed in 21 cases detected rearrangement of immunoglobulin heavy gene in 18 cases, with none having detectable Bcl-2 gene rearrangement. These data indicate that similar to DLBCL, the cell origin of neoplastic cells in THRLBCL is composed of a heterogeneous group of proliferating peripheral B cells, with only some cases originating from germinal center B cells and others derived from heterogeneous origins. Lack of Bcl-2 gene rearrangements seems to argue against a possible progression from preexisting follicular lymphoma. Thus, the normal counterpart of the neoplastic cells cannot at this time be the sole basis for the subclassification of THRLBCL.
  • H. Rojas, Jin Wang, DR Chase, J Wang. "Pathologic Quiz Case - a 46-year-old Woman Presented with One-Day History of Abdominal Pain with Resultant Intestinal Obstruction." Arch Pathol Lab Med 129.2 (2005): e44-e46. ( 1/2005 ) Link...
  • S Syed, BY Wat, J Wang. "Pathologic Quiz Case - A 55-year-old Male with Chronic Maxillary Sinusitis." Arch Pathol Lab Med 129.3 (2005): e84-e86. ( 1/2005 ) Link...
  • J. Rittenbach, J Wang. "Pathologic Quiz Case - A 12-year-old Male Heart Transplant Recipient with Cervical Lymphadenopathy." Arch Pathol Lab Med 129. (2005): 807-808. ( 1/2005 ) Link...
  • J. Wang, S. Pitman, E Rowsell, JD Cao, Q Huang. "What is Anaplastic Large Cell Lymphoma - Authors'' Reply Letter." Am J Surg Pathol 28.12 (2004): 1664-1667. ( 7/2004 )
  • Syed SP, Chase DR, Jun Wang. "Pathologic quiz case: a 63-year-old renal transplant recipient with a sore throat. Posttransplantation lymphoproliferative disorder, plasmacytoma type, with prominent Russell body formation." Archives of Pathology and Laboratory Medicine 128.6 (2004): e76-e78. ( 6/2004 ) Link...
  • J Wang; LM Weiss; B Hu; PG Chu; V Rausei-Mills, C Zuppan, D Felix, DR Chase. "Usefulnes of Immunohistochemistry in Delineating Renal Spindle Cell Tumors - A Retrospective Study of 31 Cases." Histopathology 44.5 (2004): 462-471. ( 5/2004 ) Link...
    AIMS: To assess the usefulness of immunohistochemistry in delineating tumour diagnoses on a series of morphologically diagnosed renal spindle cell tumours (RSCTs). METHODS AND RESULTS: Formalin-fixed paraffin-embedded tissues from 31 morphologically diagnosed tumours were reinterpreted in light of newly obtained immunohistochemical information. By morphology, six had originally been classified as sarcomatoid carcinoma, five as spindle cell tumour (NOS), four as sarcoma (NOS), three as leiomyoma, three as leiomyosarcoma, and one each as fibrous polyp, hamartoma, neurilemmoma, mesoblastic nephroma, medullary fibroma, angiomyolipoma, haemangiopericytoma, malignant rhabdoid tumour, malignant Triton tumour, and carcinosarcoma. The application of immunohistochemistry verified the original diagnosis in 18 cases (18/31, 58%), confirming the diagnosis of sarcomatoid renal carcinoma (4/6), leiomyoma (2/3), leiomyosarcoma (3/3), sarcoma (NOS) (2/4), carcinosarcoma (1/1), malignant rhabdoid tumour (1/1), malignant Triton tumour (1/1), fibrous polyp (1/1), mesoblastic nephroma (1/1), hamartoma (1/1), and angiomyolipoma (1/1). Different tumour designations were suggested in 13 cases (13/31, 42%), including carcinosarcoma, sarcoma (NOS), leiomyosarcoma, solitary fibrous tumour, monomorphic/biphasic angiomyolipoma, endometrial stromal tumour, and congenital mesoblastic nephroma. CONCLUSIONS: Our data indicate that although morphology is most important in formulating the initial differential diagnosis, the addition of immunohistochemistry is vital in arriving at the correct classification of RSCTs.
  • S. Pitman, E.H. Rowsell, J.D. Cao, Q. Huang, Jun Wang. "Anaplastic large cell lymphoma associated with Epstein-Barr virus following cardiac transplant." American Journal of Surgical Pathology 2004;28:410-415. ( 3/2004 ) Link...
    Posttransplantation lymphoproliferative disorders (PTLDs) eventually occur in approximately 5% of all organ transplant recipients. Most of cases are B-cell proliferations associated with the Epstein-Barr virus (EBV). T-cell PTLDs are relatively rare, although some estimate that up to 14% of posttransplantation malignant lymphomas are T-cell lymphomas even though only a few of these cases are described in the literature. A literature review found only 77 cases of T-cell PTLD, including 1 case following cardiac transplant, 15 cases associated with EBV, and only 1 case of anaplastic large cell lymphoma (ALCL). This single ALCL case followed a liver transplant, was of the T-cell phenotype, and was EBV negative. In this report, we describe a 14-year-old male who developed an EBV-positive, T-cell PTLD of the ALCL subtype after a period of 14 years following cardiac transplant. Immunohistochemical staining established the T-cell origin of the neoplasm with strong expression of CD45, CD3, CD43, and CD2 and also showed expression of CD30 consistent with the histologic features that suggested ALCL. EBER in situ hybridization detected the presence of the EBV. Polymerase chain reaction analysis for T-cell receptor-gamma gene rearrangements confirmed the T-cell lineage of this lymphoma. To our knowledge, this is the first reported case of an EBV-positive T cell lymphoma of the anaplastic large cell subtype following organ transplant.
  • L.W. McPhaul, J. Wang, E.M. Hol, M.A. Sonnemans, N. Riley, V. Nguyen, Q.X. Yuan, Y.H. Lue, F.W. van Leeuwen, and S.W. French. Molecular Misreading of Ubiquitin-B Gene and Hepatic Mallory Body Formation (Cover page article, co-first authorship). Gastroenterology. 2002;122:1878-1885. ( 6/2002 )
    Background & Aims: Molecular misreading of the ubiquitin B gene has been documented in the cerebral cortex of patients with Alzheimer’s disease and Down syndrome. This novel process consists of the unfaithful conversion of genomic information into aberrant transcripts and its subsequent translation into +1 proteins. Methods: Because Mallory bodies (MBs) also contain ubiquitinated proteins, we stained 11 autopsied and 6 biopsied MB-containing livers from patients with steatohepatitis with an antibody to ubiquitin+1 to look for the presence of mutant (ubiquitin +1) protein. Antibodies to wild-type ubiquitin were used to document the presence of MBs in all cases. Results: Ubiquitin+1 immunoreactivity was detected in all MB-containing livers with steatohepatitis; no ubiquitin+1 immunoreactivity was found in 13 MB-free liver controls. A subpopulation (about one third of the MBs) of the MB-containing hepatocytes in autopsied livers showed ubiquitin+1 immunoreactivity (i.e., ubiquitin and ubiquitin+1 colocalized in MBs). MBcontaining liver biopsy specimens showed colocalization of ubiquitin and ubiquitin+1 in every MB. Western blot analysis showed an ubiquitin+1 band of 11 kilodaltons. Molecular misreading of the ubiquitin B gene ( DGU) was shown in one of the livers, which contained numerous MBs using an expression cloning strategy. Conclusions: The results showed that molecular misreading of the ubiquitin B gene occurred in hepatocytes in virtually all of the MB-containing livers tested. Ubiquitin+1 protein was only found within the MBs and therefore may act by interfering with the degradation of the MBs because ubiquitin+1 may inhibit proteolytic function of the proteasome.
  • Jun Wang, Weiss LM, Chang KL, Slovak ML, Gaal K, Forman SJ, Arber DA.. "Diagnostic utility of bilateral bone marrow examination: significance of morphologic and ancillary technique study in malignancy." Cancer 2002;94:1522-1531. ( 3/2002 ) Link...
    BACKGROUND: To retrospectively evaluate the significance of morphologic examination and ancillary studies performed on bilateral bone marrow biopsy specimens, 1864 bone marrow samples were studied. METHODS: Bilateral bone marrow biopsy specimens included 883 specimens that were evaluated for involvement by non-Hodgkin lymphoma (NHL); 381 specimens that were evaluated for involvement by carcinoma (CA); 362 specimens that were evaluated for involvement by Hodgkin disease (HD); 94 specimens that were evaluated for involvement by sarcoma (SA); 56 specimens that were evaluated for involvement by multiple myeloma (MM); 53 specimens that were evaluated for involvement by acute and chronic leukemia, myelodysplasia, and/or myeloproliferative disorders (LEUK); and 35 specimens that were evaluated for other reasons. RESULTS: Of all 1864 specimens, 410 samples (22.0%) were positive for disease, including 77% of MM samples, 58% of LEUK samples, 29.6% of NHL samples, 14% of SA samples, 9.9% of HD samples, and 6.8% of CA samples. A discrepancy between the left and right sides was identified in 48 specimens (11.7% of positive samples). The discrepancy rate was 39% for HD samples, 29% for SA samples, 23% for CA samples, and 9.2% for NHL samples. No morphologic discrepancies between bilateral samples were found in MM samples or LEUK samples. Bilateral flow cytometric studies (n = 113 samples) were positive in 11 samples (9.7%; all morphologically positive), with two discrepancies detected between bilateral samples. Bilateral cytogenetic studies (n = 74 samples) were positive in 5 samples (7%), and there were no discrepancies. Bilateral molecular studies (n = 16 samples) were positive in 7 samples (44%), and there were 3 discrepancies. CONCLUSIONS: Bilateral morphologic evaluation is useful in the evaluation of patients with NHL, HD, CA, and SA and is not indicated for patients with acute or chronic leukemia, myelodysplasia, MM, and other diseases. Bilateral flow cytometric or cytogenetic studies of bone marrow did not provide additional information in this population to justify bilateral samples. The role of bilateral molecular analysis needs to be defined further, but pooled samples for molecular studies may be adequate. Copyright 2002 American Cancer Society.
  • Jun Wang, Sun NC, Nozawa Y, Arber DA, Chu P, Chang KL, Weiss L. "Histological and immunohistochemical characterization of extranodal diffuse large-cell lymphomas with prominent spindle cell features." Histopathology 2001;39:476-481. ( 11/2001 ) Link...
    AIMS: To describe five cases of diffuse large-cell lymphoma with prominent spindle cell components involving skin, nasal-ocular mucosa, and soft tissue. Because of the spindle cell morphology, such cases must be differentiated from true sarcomas arising in or metastasizing to soft tissue, skin, bone, lymph node, or other organs and sites. METHODS AND RESULTS: Formalin-fixed paraffin-embedded archival tissue from five consultation cases of diffuse large-cell lymphoma with prominent spindle cell features involving the skin, nasal-ocular mucosa, and soft tissue in three male and two female patients was studied by histology and immunohistochemistry. Clinicopathological findings were also reviewed for all the patients. By morphology, initial evaluation of the cases suggested spindle cell sarcoma in two cases, inflammatory pseudotumour in one case, large-cell lymphoma in another case, and one case was considered suspicious for malignant lymphoma. Immunohistochemistry demonstrated a B-cell lineage in four of the spindle cell lesions, with a diagnosis of primary cutaneous CD30+ anaplastic large cell lymphoma made for the fifth case. Four of five cases also showed actin reactivity. CONCLUSIONS: Although extremely rare, lymphomas with prominent spindle cell morphology can be encountered in daily surgical pathology practice, and should be included in the differential diagnosis of spindle cell lesions in skin and soft tissue. The observed actin reactivity in four of the five spindle cell lymphomas may lead to a misdiagnosis of leiomyosarcoma if lymphoid markers are not included in the immunohistochemical panel.
  • Jun Wang, D. Arber, K. Frankel, L.M. Weiss. "Large solitary fibrous tumor of the kidney: report of two cases and review of the literatur." American Journal of Surgical Pathology 2001;25:1194-1199. ( 9/2001 ) Link...
    Solitary fibrous tumors are spindle cell neoplasms frequently arising in the serosal surface as well as a variety of other sites. We report two cases of large solitary fibrous tumor arising in the kidney, clinically thought to be renal cell carcinoma, in 41- and 72-year-old men. Although large in size (13.0 and 14.0 cm in greatest dimension, respectively), both lesions were well circumscribed and composed of a mixture of spindle cells and dense collagenous bands with no areas of necrosis or cystic changes noted macroscopically or microscopically. Immunohistochemical studies revealed reactivity for vimentin, CD34, collagen IV, and bcl-2 protein in both cases, with no staining for keratin, S-100 protein, or muscle markers, confirming the diagnosis of solitary fibrous tumor of the kidney. Solitary fibrous tumor of the kidney is rare but may present as a large mass that may be clinically confused with carcinoma or sarcoma.
  • Nozawa Y, Jun Wang, Weiss LM, Kikuchi S, Hakozaki H, Abe M. . "Diffuse large B-cell lymphoma with spindle cell f." Histopathology 2001;38:177-178. ( 2/2001 ) Link...
  Scholarly Journals--Accepted
  • J. Kerstetter, J. Wang. Adult Orbital Xanthogranulomatous Disease: A Review with Emphasis on Etiology, Systemic Associations, Diagnostic Tools, and Treatment. Dermatology Clinics. 2015 In press ( 6/2015 )
  • H. Zhang, A. Kherapour, E.H. Rowsell, C. Zuppan, L.M. Weiss, J. Wang. Cytotoxic molecular-positive Epstein-Barr virssu-associated T-cell lymphoma in a 20-month-old child: A case report and review of the literature. Accepted by J Ped Hematol Oncol. 2015 ( 6/2015 )
  Books and Chapters
  • Nora C.J. Sun, Jun. Wang, Sheila X. Zhao, Eric F. Glassy. Bone Marrow (Chapter 12) In Silverberg S.G., DeLellis R.L., Frable W.J., LiVolsi V.A., and Wick M. eds. Principles and Practice of Surgical Pathology and Cytopathology, 5th Edition, Cambridge: Cambridge University Press, 2015. ( 3/2015 )
  • Mingyi Chen, Thomas J. Semrad, Jun Wang. Diagnosis of Primary Gastrointestinal Lymphomas and Mimics. In Oncology – Theory & Practice.  Brisbane, Australia: iConcept Press Ltd, 2013. ( 9/2013 )
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