Loma Linda University

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Carlos Casiano, PhD
Professor, Basic Sciences
School of Medicine
Professor, Medicine
School of Medicine
Member, Microbiology&Molecular Gen, SM, Faculty of Graduate Studies
Publications    Scholarly Journals--Published
  • Almaguel FG,  Liu JW,  Pacheco FJ,  De Leon DD,  Casiano CA, and De Leon M (2010).  Lipotoxicity-mediated cell dysfunction and death involves lysosomal membrane permeabilization and cathepsin L activity.   Brain Research 1318:133-43.   ( 2/2010 )
     
  • Mediavilla-Varela M, Pacheco FJ, Almaguel F, Daniels TR, Padilla A, Perez J, Leoh LS, Sahakian E, Wall NR, Lilly MB, De Leon M, and Casiano CA (2009).  Docetaxel-induced prostate cancer cell death involves concomitant activation of caspase and lysosomal pathways and is attenuated by LEDGF/p75.  Molecular Cancer 8:68. ( 10/2009 )
     
  • Almaguel FG, Liu JW, Pacheco FJ, Casiano CA, De Leon MA (2009) Activation and reversal of lipotoxicity in neurons following exposure to palmitic acid.  J. Neuroscience Research 87:1207-18. ( 2/2009 )
     
  •  Brown-Bryan TA, Leoh LS, Ganapathy V, Pacheco FJ, Mediavilla-Varela M, Fillipova M, Linkhart T, Gijsberg R, Debyser Z, and Casiano CA (2008). Alternative splicing and caspase-mediated cleavage generate antagonistic variants of the stress response oncoprotein LEDGF/p75.  Molecular Cancer Research 6:1293-1307. ( 8/2008 )
     
  • Sheets SM, Robles A, Casiano CA, and Fletcher HM.  (2008) Survival strategies: P. gingivalis gingipain-dependent interactions with the host.   Frontiers in Bioscience 13:3215-3238. ( 5/2008 )
     
  • Sheets SM, Potempa J, Travis J, Fletcher HM, Casiano CA. (2006) "Gingipains from Porphyromonas gingivalis W83 synergistically disrupt endothelial cell adhesion and can induce caspase-independent apoptosis." Infection and Immunity 74: 5667-5678. ( 10/2006 )
    We have shown previously that gingipains from Porphyromonas gingivalis W83 can induce cell detachment, cell adhesion molecule (CAM) cleavage, and apoptosis in endothelial cells; however, the specific roles of the individual gingipains are unclear. Using purified gingipains, we determined that each of the gingipains can cleave CAMs to varying degrees with differing kinetics. Kgp and HRgpA work together to quickly detach endothelial cells. Interestingly, in the absence of active caspases, both gingipain-active W83 extracts and purified HRgpA and RgpB induce apoptotic morphology, suggesting that the gingipains can induce both caspase-dependent and caspase-independent apoptosis. Using z-VAD-FMK to inhibit Kgp activity and leupeptin to inhibit Rgp activity in gingipain-active W83 extracts, we investigated the relative significance of the synergistic role of the gingipains. z-VAD-FMK or leupeptin delayed, but did not inhibit, cell detachment induced by gingipain-active W83 extracts or purified gingipains. There was partial cleavage of N-cadherin and cleavage of VE-cadherin was not inhibited. Degradation of integrin beta1 was inhibited only in the presence of z-VAD-FMK. These results further clarify the role P. gingivalis plays in tissue destruction occurring in the periodontal pocket.
  • Casiano CA, Mediavilla-Varela M, Tan EM. (2006) "Tumor-associated antigen arrays for the serological diagnosis of cancer ." Molecular and Cellular Proteomics 5: 1745-1759. ( 10/2006 )
    The recognition that human tumors stimulate the production of autoantibodies against autologous cellular proteins called tumor-associated antigens (TAAs) has opened the door to the possibility that autoantibodies could be exploited as serological tools for the early diagnosis and management of cancer. Cancer-associated autoantibodies are often driven by intracellular proteins that are mutated, modified, or aberrantly expressed in tumor cells and hence are regarded as immunological reporters that could help uncover molecular events underlying tumorigenesis. Emerging evidence suggests that each type of cancer might trigger unique autoantibody signatures that reflect the nature of the malignant process in the affected organ. The advent of novel genomic, proteomic, and high throughput approaches has accelerated interest in the serum autoantibody repertoire in human cancers for the discovery of candidate TAAs. The use of individual anti-TAA autoantibodies as diagnostic or prognostic tools has been tempered by their low frequency and heterogeneity in most human cancers. However, TAA arrays comprising several antigens significantly increase this frequency and hold great promise for the early detection of cancer, monitoring cancer progression, guiding individualized therapeutic interventions, and identification of novel therapeutic targets. Our recent studies suggest that the implementation of TAA arrays in screening programs for the diagnosis of prostate cancer and other cancers should be preceded by the optimization of their sensitivity and specificity through the careful selection of the most favorable combinations of TAAs
  • Pacheco FJ, Servin JA, Dang D, Kim J, Molinaro C, Daniels T, Brown-Bryan TA, and Casiano CA . (2005) "Involvement of lysosomal cathepsins in the cleavage of topoisomerase I during necrotic cell death." Arthritis & Rheumatism 52.: 2133-2145. ( 7/2005 )
     
  • Filippova M, Bryan-Brown T, Casiano CA, and Duerksen-Hughes PJ. (2005) "The human papillomavirus 16 E6 protein can render cells either sensitive or resistant to TNF: effect of dose." Cell Death and Differentiation 12: 1622-1635. ( 6/2005 )
     
  • Shi F, Zhang JY, Rearden A, Elliot M, Nachtsheim D, Daniels T, Casiano CA, Chan EKL, and Tan EM. (2005) "Preferential humoral immune response to p90 and p62 in prostate cancer." Prostate 63: 252-258. ( 1/2005 )
     
  • Daniels T, Zhang JY, Gutierrez I, Elliott ML, Yamada B, Heeb MJ, Sheets S, Wu X, and Casiano CA (2005). "Antinuclear autoantibodies in prostate cancer: immunity to LEDGF/p75, a survival protein highly expressed in prostate tumors and cleaved during apoptosis." Prostate 62: 14-26. ( 1/2005 )
     
  • Sheets SM, Potempa J, Travis J, Casiano CA, and Fletcher HM . (2005) "Gingipains from Porphyromonas gingivalis W83 induce cell adhesion molecule cleavage and apoptosis in endothelial cells ." Infection and Immunity 73: 1543-1552. ( 1/2005 )
     
  Scholarly Journals--Accepted
  • Fritzler MJ,  Rattner JB, Luft LM, Edworthy SM, Casiano CA, Peebles C, Mahler M (2010).  Historical perspectives on the discovery of autoantibodies to centromere proteins (CENP) and the emerging importance of antibodies to CENP-F.   Autoimmunity Reviews  (in press) ( 10/2010 )
     
  • Basu A, Banerjee H, Rojas H, Martinez SR, Roy S, Jia Z, Lilly MB, De Leon M, Casiano CA (2010).  Differential expression of peroxiredoxins in prostate cancer: consistent upregulation of PRDX3 and PRDX4.  Prostate (In press).   ( 10/2010 )
     
  Books and Chapters
  • Leoh LS, Mediavilla-Varela M, Basu A, and Casiano CA (2009).  Autoantibodies to LEDGFp75/DFS70: natural autoantibodies or sensors of an augmented state of cellular oxidative stress?  In “From Pathogenesis to Therapy of Autoimmune Diseases”. Autoimmunity, Autoantigens, and Autoantibodies 6:135-156.  Ed. Conrad K, Chan EKL, Fritzler MJ, Humbel RL, von Landenberg P, Shoenfeld Y.  PABST Science Publishers, Lengerich, Germany.   ( 9/2009 )
     
  • Mediavilla-Varela,  Leoh LS,  Basu A, Ganapathy V, Casiano CA (2007).   LEDGFp75/DFS70, a stress response autoantigen with multiple functions and broad clinical relevance.   In “From Ethiopathogenesis to the Prediction of Autoimmune Disease: Relevance of Autoantibodies” Autoimmunity, Autoantigens, and Autoantibodies 5:147-165.  Ed. Conrad K and Sack U.  PABST Science Publishers, Lengerich, Germany. ( 9/2007 )
     
  • Casiano CA and Pacheco FJ. (2006)  Autoimmunity and Cell Death. Autoantibodies and Autoimmunity: Molecular Mechanisms in Health and Disease: Wiley-VCH Verlag GmbH, Weinheim, Germany. 107 - 137 ( 1/2006 )
     
  • Casiano CA and Pacheco FJ. (2005) Autoimmunity and Cell Death. In "Autoantibodies and Autoimmunity: Molecular Mechanisms in Health and Disease". Weinheim, Germany: WILEY-VCH Verlag GmbH. 107 - 137 ( 1/2005 )
     
  • Brown-Bryan TA, Daniels T, and Casiano CA. (2004) Autoimmunity to the survival protein LEDGF/p75 in inflammatory disorders and cancer: clinical and biological aspects. In Autoimmunity, autoantigens, and autoantibodies . Lengerich, Germany: PABST Science Publishers. 209 - 227 ( 9/2004 )